The pruned nodes (in red) represent entire regions of space that the algorithm never examines. The points inside those regions are never checked. Compare the "Nodes Visited" count to the total number of points. The quadtree is doing far less work than a brute-force scan.
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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?
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